{"product_id":"recombinant-tryptase-cell-digest","title":"Recombinant tryptase cell digest","description":"\u003cp\u003eProduct Name Name:Recombinant tryptase cell digest Product Overview Trypsin, short for trypsin, is a serine hydrolase that cuts off the carboxyl terminus in lysine and arginine in polypeptide chains. Trypsin digests are used in tissue cell extraction and culture, as well as in in vitro cell culture, to hydrolyze intercellular proteins and dissociate tissues or cells into individual cells. Conventional trypsin is obtained from the pancreas of animals such as pigs and cattle. The source of traditional trypsin and the characteristics of the production process determine that it will inevitably contain impurities of animal origin as well as potential viral contamination. This product uses recombinant porcine pancreatin to replace traditional pancreatin. It does not use any animal-derived raw materials in the production and preparation process, and ensures the purity and viability of recombinant porcine pancreatin through multiple rounds of purification. It can not only replace the function of traditional trypsin, but also can be used for the digestion of special cells or tissues that strictly require the use of no animal source. Application Fields Accelerates hydrolysis of intercellular junction proteins and enhances digestion Operation steps 1. This product can be taken out at 4°C and used for cell digestion directly, the digestion temperature can be selected according to the needs of room temperature or incubator digestion, but please do not put this product directly at 37°C preheating, the temperature repeatedly rise and fall on the enzyme activity has a certain effect. 2. After receiving the product, it can be stored in separate packages as needed; if it needs to be stored at -20°C, avoid repeated freezing and thawing. 3. The digestion time is different for different cell lines or tissues, please confirm the optimal digestion time to avoid over-digestion caused by too long digestion time, which may affect the cell activity and status. 4. This product is sterile and ready-to-use, and has been tested for mycoplasma and endotoxin, so please pay attention to aseptic operation during use. 5. For your health and safety, please wear lab coat and gloves during operation. Related Information Please e-mail for the detailed characterization data. E-mail:sales@xfnano.com\u003c\/p\u003e","brand":"xfnano","offers":[{"title":"100 mL Swe recombinant pancreatic enzyme cell digestion solution (containing EDTA, containing phenol red) 105896 \/ Store at 4°C refrigerated \/ 365days","offer_id":57280516096378,"sku":"105896","price":18.75,"currency_code":"USD","in_stock":true},{"title":"100 mL Swe recombinant pancreatic enzyme cell digestion solution (containing EDTA, without phenol red) 105938 \/ Store at 4°C refrigerated \/ 365days","offer_id":57280516129146,"sku":"105938","price":18.75,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0907\/3606\/6938\/files\/1_27871cef-ce01-415a-9a0e-d29d5e516d17.png?v=1780024497","url":"https:\/\/chinanano.com\/es\/products\/recombinant-tryptase-cell-digest","provider":"xfnano","version":"1.0","type":"link"}