Recombinant Human IL-17A Protein (His-tag)

DESCRIPTION Gene Name Synonym IL-17A, Il17a, Ctla8, Il17 Species Human Accession Q16552 Source HEK293 cells SPECIFICATIONS Amino acid sequence Gly24-Ala155 is expressed with a 10×His tag at the C terminus Molecular Weight 15.1 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg-1 Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Data SDS-PAGE Bioactivity Recombinant Human IL-17A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 18-22 kDa. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNF-α. The ED50 for this effect is ≤11.1 ng/mL.