Recombinant Human TNF-alpha Protein

DESCRIPTION Gene Name Synonym TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin Species Human Accession P01375 Source HEK293 cells SPECIFICATIONS Amino acid sequence Val77-Leu233 is expressed with a 10×His tag at the N terminus Molecular Weight 18.8 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg-1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. Data SDS-PAGE Bioactivity Active Recombinant Human TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue.