{"product_id":"recombinant-human-tnf-alpha-protein","title":"Recombinant Human TNF-alpha Protein","description":"\u003cp\u003eDESCRIPTION Gene Name Synonym TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin Species Human Accession P01375 Source HEK293 cells SPECIFICATIONS Amino acid sequence Val77-Leu233 is expressed with a 10×His tag at the N terminus Molecular Weight 18.8 kDa Purity \u0026gt;95.0% as determined by SDS-PAGE Endotoxin \u0026lt;5 EU mg-1 Formulation Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. Data SDS-PAGE Bioactivity Active Recombinant Human TNF-alpha Protein was determined by SDS-PAGE with Coomassie Blue.\u003c\/p\u003e","brand":"xfnano","offers":[{"title":"10 μg TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin 106238 \/ Store at -20°C \/ 365days","offer_id":57280528089466,"sku":"106238","price":125.0,"currency_code":"USD","in_stock":true},{"title":"100 μg TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin 106239 \/ Store at -20°C \/ 365days","offer_id":57280528122234,"sku":"106239","price":640.63,"currency_code":"USD","in_stock":true},{"title":"500 μg TNF-alpha, TNFα, DIF, TNFA, TNFSF2, cachexin, cachectin 106240 \/ Store at -20°C \/ 365days","offer_id":57280528155002,"sku":"106240","price":1723.44,"currency_code":"USD","in_stock":true},{"title":"10 μg Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF 106241 \/ Store at -20°C \/ 365days","offer_id":57280528187770,"sku":"106241","price":60.94,"currency_code":"USD","in_stock":true},{"title":"100 μg Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF 106242 \/ Store at -20°C \/ 365days","offer_id":57280528220538,"sku":"106242","price":268.75,"currency_code":"USD","in_stock":true},{"title":"500 μg Tumor Necrosis Factor, TNFA, TNFSF2, Cachectin, Cytotoxin, Differentiation-inducing factor, DIF 106243 \/ Store at -20°C \/ 365days","offer_id":57280528253306,"sku":"106243","price":745.31,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0907\/3606\/6938\/files\/1_27871cef-ce01-415a-9a0e-d29d5e516d17.png?v=1780024497","url":"https:\/\/chinanano.com\/products\/recombinant-human-tnf-alpha-protein","provider":"xfnano","version":"1.0","type":"link"}