{"product_id":"red-blood-cell-lysis-buffer","title":"Red Blood Cell Lysis Buffer","description":"\u003cp\u003eProduct Name Name:Red Blood Cell Lysis Buffer Product Overview Red Blood Cell Lysis Buffer, or ACK Lysis Buffer, is a classic lysate used to remove red blood cells, fully removing red blood cells without damaging nucleated cells. Tissue cells obtained by lysis of erythrocyte lysis buffer do not contain erythrocytes, and can be further used for primary culture, cell fusion, flow cytometric analysis, isolation and extraction of nucleic acids and proteins. The basic principle is to use the principle of lysis of red blood cells by utilizing the concentration difference of intracellular osmotic pressure which leads to the swelling of cell membrane. The main components include ammonium chloride, potassium bicarbonate, EDTA-Na2, because ammonium ions can not pass through the cell membrane, while other ions can pass through, resulting in the difference in ion concentration between inside and outside the cell, the external water diffusion into the cell, so that the red blood cell expansion, to achieve the effect of lysis. This product is filtered by 0.2 μm filter membrane to remove bacteria. Application Fields Bacteria removal by 0.2 μm membrane filtration. Classical lysate for removing red blood cells without damaging nucleated cells, the main active ingredient is ammonium chloride. Operation steps 1. This product has been filtered to remove bacteria, please pay attention to aseptic operation when using to avoid bacterial contamination. 2. If the subsequent test is used for cell culture, the operation should be completed in the ultra-clean bench, and pay attention to the aseptic operation, so as to avoid cell contamination and affect the cell culture. 3. After centrifugal washing, if a very small amount of erythrocytes are found, the test can be continued without affecting the subsequent test. 4. Please wear lab coat and disposable gloves during operation. Related Information Please e-mail for the detailed characterization data. E-mail:sales@xfnano.com\u003c\/p\u003e","brand":"xfnano","offers":[{"title":"500 mL Red blood cell lysis buffer 105894 \/ Store at 4°C refrigerated \/ 365days","offer_id":57280516030842,"sku":"105894","price":36.72,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0907\/3606\/6938\/files\/1_27871cef-ce01-415a-9a0e-d29d5e516d17.png?v=1780024497","url":"https:\/\/chinanano.com\/products\/red-blood-cell-lysis-buffer","provider":"xfnano","version":"1.0","type":"link"}