Recombinant Human IL-4 Protein

DESCRIPTION Gene Name Synonym IL-4, IL4, BCGF1, BSF1 Species Human Accession P05112 Source HEK293 cells SPECIFICATIONS Amino acid sequence His25-Ser153 Molecular Weight 15 kDa Purity >95.0% as determined by SDS-PAGE Endotoxin <5 EU mg-1 Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Reconstitution Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. STORAGE Stability and Storage 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. BACKGROUND Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 was first identified as a T cell-derived growth factor for B cells. In addition to T cells, IL-4 is produced by innate lymphocytes, such as NTK cells, and myeloid cells, such as basophils and mast cells. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. Data SDS-PAGE Bioactivity Active Recombinant Human IL-4 Protein was determined by SDS-PAGE with Coomassie Blue.